Online UV-visible spectroscopy and multivariate curve resolution as powerful tool for model-free investigation of laccase-catalysed oxidation.

@article{Kandelbauer2008OnlineUS,
  title={Online UV-visible spectroscopy and multivariate curve resolution as powerful tool for model-free investigation of laccase-catalysed oxidation.},
  author={Andreas Kandelbauer and Waltraud Kessler and R. W. Kessler},
  journal={Analytical and bioanalytical chemistry},
  year={2008},
  volume={390 5},
  pages={1303-15}
}
The laccase-catalysed transformation of indigo carmine (IC) with and without a redox active mediator was studied using online UV-visible spectroscopy. Deconvolution of the mixture spectra obtained during the reaction was performed on a model-free basis using multivariate curve resolution (MCR). Thereby, the time courses of educts, products, and reaction intermediates involved in the transformation were reconstructed without prior mechanistic assumptions. Furthermore, the spectral signature of a… CONTINUE READING