One-tube post-PCR fluorescent labeling of DNA fragments.

@article{Inazuka1996OnetubePF,
  title={One-tube post-PCR fluorescent labeling of DNA fragments.},
  author={M Inazuka and Tomoko Tahira and Kenshi Hayashi},
  journal={Genome research},
  year={1996},
  volume={6 6},
  pages={551-7}
}
A method for fluorescent postlabeling of PCR products has been developed. The method uses Klenow fragment of DNA polymerase I that exchanges the 3'-terminal residue of PCR-amplified DNA fragment for fluorescent nucleotides. All reactions, including PCR, are performed in one tube simply by successive addition of reagents. The products can be applied directly to fluorescence-based automated DNA sequencers without purification for either length determination in denaturing electrophoresis or… CONTINUE READING
Highly Cited
This paper has 50 citations. REVIEW CITATIONS

5 Figures & Tables

Topics

Statistics

01020'00'02'04'06'08'10'12'14'16'18
Citations per Year

51 Citations

Semantic Scholar estimates that this publication has 51 citations based on the available data.

See our FAQ for additional information.