One-tube post-PCR fluorescent labeling of DNA fragments.

  title={One-tube post-PCR fluorescent labeling of DNA fragments.},
  author={M Inazuka and Tomoko Tahira and Kenshi Hayashi},
  journal={Genome research},
  volume={6 6},
A method for fluorescent postlabeling of PCR products has been developed. The method uses Klenow fragment of DNA polymerase I that exchanges the 3'-terminal residue of PCR-amplified DNA fragment for fluorescent nucleotides. All reactions, including PCR, are performed in one tube simply by successive addition of reagents. The products can be applied directly to fluorescence-based automated DNA sequencers without purification for either length determination in denaturing electrophoresis or… CONTINUE READING
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