One-step typing of Epstein-Barr virus by polymerase chain reaction: predominance of type 1 virus in Japan.

@article{Kunimoto1992OnestepTO,
  title={One-step typing of Epstein-Barr virus by polymerase chain reaction: predominance of type 1 virus in Japan.},
  author={Masaru Kunimoto and Shinji Tamura and Toshihide Tabata and Osamu Yoshie},
  journal={The Journal of general virology},
  year={1992},
  volume={73 ( Pt 2)},
  pages={
          455-61
        }
}
The prevalence of two types of Epstein-Barr virus (EBV) in Japan was studied by using the polymerase chain reaction (PCR). The U2 region encoding EBV nuclear antigen 2 (EBNA-2) was chosen as the target of amplification. Consensus primers were synthesized from sequences common to the two types but encompassing a large stretch of deletion in the sequence of type 1 EBV. The primers were capable of amplifying both types at the same time but allowed differentiation of each type by the size of the… CONTINUE READING

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