One-step real-time RT-PCR assays for serotyping dengue virus in clinical samples

@inproceedings{Alm2015OnestepRR,
  title={One-step real-time RT-PCR assays for serotyping dengue virus in clinical samples},
  author={Erik Alm and Gunnel Lindegren and Kerstin Ingrid Falk and Nina Lagerqvist},
  booktitle={BMC infectious diseases},
  year={2015}
}
BACKGROUND Dengue is one of the leading causes of morbidity in tropical and subtropical regions and infection with any of the four dengue virus serotypes (DENV1-4) result in a wide range of clinical manifestations. Given the geographic expansion of DENV1-4, assays for serotyping are needed to be able to perform surveillance and epidemiological studies. In this study, we describe the design and validation of one-step real-time serotype-specific DENV RT-PCR assays. METHODS The DENV1, DENV2… CONTINUE READING