One-step purification of histidine-tagged profilin with high purity and yield by using metal precipitation.

@article{Sankian2007OnestepPO,
  title={One-step purification of histidine-tagged profilin with high purity and yield by using metal precipitation.},
  author={Mojtaba Sankian and Mostafa Yousefi and Nazanin Pazouki and Abdolreza Varasteh},
  journal={Biotechnology and applied biochemistry},
  year={2007},
  volume={47 Pt 4},
  pages={
          185-9
        }
}
A simple one-step method for the purification of recombinant His-tagged profilin from the bacterial cell lysate is reported. Noting the greater ease with which hexahistidine-tagged proteins can be metalprecipitated compared with unwanted protein impurities, we investigated the effect of lysis-buffer additives and optimization of other conditions to recover selectively desired proteins in a one-step metal precipitation without using biopolymers. Purification of the His-tagged melon (Cucumis melo… CONTINUE READING
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