One-step purification and characterization of an intracellular β-glucosidase from Metschnikowia pulcherrima

@article{GonzlezPombo2008OnestepPA,
  title={One-step purification and characterization of an intracellular β-glucosidase from Metschnikowia pulcherrima},
  author={Paula Gonz{\'a}lez-Pombo and Gabriel Perez and Francisco Carrau and Jose M Guisan and Francisco Batista-Viera and Beatriz M. Brena},
  journal={Biotechnology Letters},
  year={2008},
  volume={30},
  pages={1469-1475}
}
A collection of 60 non-Saccharomyces yeasts isolated from grape musts in Uruguayan vineyards was screened for β-glucosidase activity and Metschnikowia pulcherrima was the best source of this enzyme activity. Its major β-glucosidase was successfully purified to homogeneity by ion-exchange chromatography on amino-agarose gel. The enzyme exhibited an optimum catalytic activity at 50°C and pH 4.5 and was active against (1 → 4)-β and (1 → 2)-β glycosidic linkages. In spite of preserving 100% of its… CONTINUE READING
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