One-step multiplex RT-PCR for detection and subtyping of swine influenza H1, H3, N1, N2 viruses in clinical samples using a dual priming oligonucleotide (DPO) system.

@article{Lee2008OnestepMR,
  title={One-step multiplex RT-PCR for detection and subtyping of swine influenza H1, H3, N1, N2 viruses in clinical samples using a dual priming oligonucleotide (DPO) system.},
  author={C. Lee and B. K. Kang and D. Lee and S. H. Lyou and B. K. Park and S. K. Ann and K. Jung and D. Song},
  journal={Journal of virological methods},
  year={2008},
  volume={151 1},
  pages={
          30-4
        }
}
  • C. Lee, B. K. Kang, +5 authors D. Song
  • Published 2008
  • Biology, Medicine
  • Journal of virological methods
  • The swine influenza virus (SIV) H1N1, H1N2, and H3N2 subtypes circulate in Korean farm. A novel multiplex RT-PCR (m-RT-PCR) was developed to detect and subtype swine influenza viruses. This m-RT-PCR assay could identify H1, H3, N1 and N2 from clinical samples in single tube reaction using DPO system. Korean SIVs are closely related to the United States influenza viruses, and primers were developed for SIV from North American viruses and recently Korean isolates. The sensitivity of the m-RT-PCR… CONTINUE READING
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