One-step intracellular triglycerides extraction and quantitative measurement in vitro.

INTRODUCTION Measurement of intracellular triglyceride content is traditionally performed using Oil Red O stain and isopropanol extraction. However, the assay is semi-quantitative and its simplicity needs improving. Therefore, a novel assay is described to address these problems. METHODS Intracellular triglycerides (TG) were extracted directly by organic… CONTINUE READING