One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces.

@article{Huang2015OnestepHC,
  title={One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces.},
  author={He Huang and Guosong Zheng and Wei-hong Jiang and Haifeng Hu and Yinhua Lu},
  journal={Acta biochimica et biophysica Sinica},
  year={2015},
  volume={47 4},
  pages={231-43}
}
The RNA-guided DNA editing technology CRISPRs (clustered regularly interspaced short palindromic repeats)/Cas9 had been used to introduce double-stranded breaks into genomes and to direct subsequent site-specific insertions/deletions or the replacement of genetic material in bacteria, such as Escherichia coli, Streptococcus pneumonia, and Lactobacillus reuteri. In this study, we established a high-efficiency CRISPR/Cas9 genome editing plasmid pKCcas9dO for use in Streptomyces genetic… CONTINUE READING
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