One-step generation of recombineering constructs by asymmetric-end ligation and negative selection.

@article{Ohtsuka2007OnestepGO,
  title={One-step generation of recombineering constructs by asymmetric-end ligation and negative selection.},
  author={M. Ohtsuka and A. Mizutani and Y. Y. Kikuti and J. Kulski and M. Sato and M. Kimura and M. Tanaka and H. Inoko},
  journal={Analytical biochemistry},
  year={2007},
  volume={360 2},
  pages={
          306-8
        }
}
Recombineering [1] or Red/ET recombination [2], a recently developed technique based on homologous recombination in Escherichia coli, is useful for the efficient production of a gene-targeting vector (TV) by modification of bacterial artificial chromosome (BAC) clones. This method requires the preparation of a linear DNA recombineering fragment, containing a selectable gene flanked by homologous regions (HRs) for recombination. The PCR method using primers containing HR sequences is the… Expand
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References

SHOWING 1-7 OF 7 REFERENCES
A highly efficient recombineering-based method for generating conditional knockout mutations.
  • 1,034
  • Highly Influential
  • PDF
Recombineering: a powerful new tool for mouse functional genomics
  • 758
  • PDF
A new logic for DNA engineering using recombination in Escherichia coli
  • 1,097
High-throughput engineering of the mouse genome coupled with high-resolution expression analysis
  • 377
  • PDF
Construction of mouse 129/Ola BAC library for targeting experiments using E14 embryonic stem cells.
  • 13
Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria.
  • 435
  • PDF
Searching for peptide ligands with an epitope library.
  • 1,907