One-step enzyme-linked immunosorbent assay (ELISA) for plasma fatty acid-binding protein.

  title={One-step enzyme-linked immunosorbent assay (ELISA) for plasma fatty acid-binding protein.},
  author={K W Wodzig and Maurice M. A. L. Pelsers and Ger J. van der Vusse and Wynand Roos and Jan F. C. Glatz},
  journal={Annals of clinical biochemistry},
  volume={34 ( Pt 3)},
To allow a more rapid determination of heart-type fatty acid-binding protein (FABP) concentration in plasma a direct non-competitive (sandwich-type) ELISA was developed which uses high-affinity monoclonal antibodies to FABP. Total performance time of the one-step immunoassay is 45 min. The standard curve was linear between 0.2-6 micrograms/L, and the within-run and between-run coefficients of variations were below 6 and 11%, respectively. The serum FABP concentration measured in 79 healthy… CONTINUE READING


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