One-step cloning and chromosomal integration of DNA.

  title={One-step cloning and chromosomal integration of DNA.},
  author={François St-Pierre and Lun Cui and David G Priest and Drew Endy and Ian B. Dodd and Keith E. Shearwin},
  journal={ACS synthetic biology},
  volume={2 9},
We describe "clonetegration", a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids. 
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