One step antibiotic disk method for obtaining axenic cultures of multicellular marine algae

  title={One step antibiotic disk method for obtaining axenic cultures of multicellular marine algae},
  author={P. A. Bradley and Donald P. Cheney and Naotsune Saga},
  journal={Plant Cell, Tissue and Organ Culture},
A simple and rapid method for obtaining axenic plant material has been devised where antibiotic-containing paper disks are applied to nutrient agarose or agar plates upon which small pieces of plant have been spread. By applying multiple disks to the agarose plate, susceptibility to a large number of antibiotics can be tested simultaneously. Clear zones are produced around those disks where contaminating bacteria are susceptible. Plant pieces are then removed from the clear zones and separately… CONTINUE READING

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