One step antibiotic disk method for obtaining axenic cultures of multicellular marine algae

@article{Bradley2004OneSA,
  title={One step antibiotic disk method for obtaining axenic cultures of multicellular marine algae},
  author={P. A. Bradley and Donald P. Cheney and Naotsune Saga},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={12},
  pages={55-60}
}
A simple and rapid method for obtaining axenic plant material has been devised where antibiotic-containing paper disks are applied to nutrient agarose or agar plates upon which small pieces of plant have been spread. By applying multiple disks to the agarose plate, susceptibility to a large number of antibiotics can be tested simultaneously. Clear zones are produced around those disks where contaminating bacteria are susceptible. Plant pieces are then removed from the clear zones and separately… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

References

Publications referenced by this paper.
Showing 1-10 of 16 references

Pure culture of algae

N Saga
1986

Pure culture of algae. [In Japanese

N Saga
Plant Biotechnology. Tokyo Kagaku Dojin, • 1986
View 3 Excerpts

Developmental studies in Porphyra. I - Blade differentiation in Porphyra perforata as expressed by morphology, enzymatic digestion, and protoplast regeneration

M Polne-Fuller, A Gibor
J Phycol • 1984
View 3 Excerpts

Plant Propagation by Tissue Culture: handbook and directory of commercial laboratories

EF George, PD Sherrington
Exegetics Ltd., Hants UK, • 1984
View 1 Excerpt

A new method for pure culture of macroscopic algae, the one step selection method

N Saga, Y Sakai
Jap J Phycol • 1982
View 2 Excerpts

Laboratory culture of Agardhiella subulata (Rhodophyta), strain A~

T Capo
Biol Bull • 1981
View 1 Excerpt

Algal sheath removal and axenic algae cultures

PM Bradley, RL Pesano
J Cell Biol • 1980
View 1 Excerpt

Bacteria induced polymorphism in an axenic laboratory strain of Ulva lactuca (Chlorophyceae)

L Provasoli, IJ Pintner
J Phycol • 1980
View 1 Excerpt

Susceptibility testing: diffusion test procedures

AL Barry, C Thornsberry
Lennette EH (ed) Manual of Clinical Microbiology, 3rd. edit. Amer Soc Microbiol, Washington DC, • 1980
View 2 Excerpts

Untersuchungen zur Sterilisation von mehrzelligen Brackwasseralgen mit Hilfe von Antibiotika, Sulfonamiden und Fungiziden

U Schiewer
Int Rev ges Hydrobiol • 1969
View 1 Excerpt

Similar Papers

Loading similar papers…