One-step analysis of protein complexes in microliters of cell lysate

Abstract

We present 'mix and measure' procedures for the analysis of protein complexes in microliters of crude human and mouse cell lysates using fluorescence correlation and crosscorrelation spectroscopy. We labeled interacting endogenous proteins by indirect immunofluorescence with all primary and secondary reagents added in one step. Especially for the screening… (More)
DOI: 10.1038/nmeth802

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