One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins.

@article{Lafontaine1996OnestepPM,
  title={One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins.},
  author={Denis L J Lafontaine and David Tollervey},
  journal={Nucleic acids research},
  year={1996},
  volume={24 17},
  pages={3469-71}
}
With the availability of the complete yeast genomic sequence, techniques which allow the rapid functional analysis of genes of interest are of increasing importance. Here we report a technique which allows the initial characterisation of genes of interest, through the construction of conditionally expressed mutations for functional analyses and the generation of epitope-tagged fusion proteins for immuno-localisation and immuno-purification, entirely by PCR.