One-step DNA fragment assembly and circularization for gene cloning.

@article{Zuo2010OnestepDF,
  title={One-step DNA fragment assembly and circularization for gene cloning.},
  author={Peijun Zuo and Bakr M. Rabie},
  journal={Current issues in molecular biology},
  year={2010},
  volume={12 1},
  pages={11-6}
}
This article describes a one-step procedure based on Taq polymerase for the precise assembly of DNA fragments into circular constructs as long as 6 kb. The only prior step needed was the amplification of the gene to be cloned and the linear vector backbone, and the whole process up to assembly and circularization lasted only 2 days, compared with the conventional method's 2 weeks. Furthermore, the final DNA construct was used to transform Escherichia coli directly without any further treatment… CONTINUE READING

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