One-pot preparation of labelled mannan–peptide conjugate, model for immune cell processing

Abstract

An efficient method for preparation of fluorescently labelled mannan–peptide glycoconjugates has been developed. After selective Dess–Martin periodinane oxidation of mannan, it was conjugated to the fluorescent label alone and a peptide with the label via reductive amination. Prepared glycoconjugates were characterised by HPSEC, FTIR-ATR and UV-VIS spectroscopy. Finally, the fluorescently labelled mannan and mannan–peptide conjugate were used for microscopic visualization of their accumulation in intracellular organelles of RAW 264.7 cells.

DOI: 10.1007/s10719-015-9644-0

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@article{Farkas2015OnepotPO, title={One-pot preparation of labelled mannan–peptide conjugate, model for immune cell processing}, author={P. Farkas and Al{\vz}beta {\vC}{\'i}{\vz}ov{\'a} and Peter Bystrick{\'y} and Lucia Paulovi{\vc}ov{\'a} and Ema Paulovi{\vc}ov{\'a} and Slavom{\'i}r Bystrick{\'y}}, journal={Glycoconjugate Journal}, year={2015}, volume={33}, pages={113-120} }