One-pot nonreductive O-glycan release and labeling with 1-phenyl-3-methyl-5-pyrazolone followed by ESI-MS analysis.

@article{Wang2011OnepotNO,
  title={One-pot nonreductive O-glycan release and labeling with 1-phenyl-3-methyl-5-pyrazolone followed by ESI-MS analysis.},
  author={Chengjian Wang and Wancui Fan and Ping Zhang and Zhongfu Wang and Linjuan Huang},
  journal={Proteomics},
  year={2011},
  volume={11 21},
  pages={4229-42}
}
A novel one-pot procedure for the nonreductive release of O-linked glycans from glycoproteins and the simultaneous derivatization of released glycans with 1-phenyl-3-methyl-5-pyrazolone (PMP) is described. Unlike the traditional reductive β-elimination, which produces alditols, this new method employs PMP/ammonia aqueous solution as the reaction medium. The O-glycans are released from glycoproteins and derivatized with PMP nonreductively, specifically, and quantitatively. Samples can be easily… CONTINUE READING

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