One-pot construction of mediatorless bi-enzymatic glucose biosensor based on organic-inorganic hybrid.
@article{Manesh2010OnepotCO, title={One-pot construction of mediatorless bi-enzymatic glucose biosensor based on organic-inorganic hybrid.}, author={K. M. Manesh and P. Santhosh and S. Uthayakumar and A. Gopalan and K. Lee}, journal={Biosensors & bioelectronics}, year={2010}, volume={25 7}, pages={ 1579-86 } }
A new methodology for the fabrication of bienzymatic amperometric glucose biosensor based on the use of an organic-inorganic hybrid is presented. The fabrication involves a self-assembly directed one-pot electrochemical process. Bi-enzymes, horseradish peroxidase (HRP) and glucose oxidase (GOx) are immobilized into the porous and electroactive silica-polyaniline hybrid composite through electrochemical polymerization of N[3-(trimethoxysilyl)propyl]aniline in the presence of enzymes. The… CONTINUE READING
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