One-pot biocatalytic amine transaminase / acyl transferase cascade for aqueous formation of amides from aldehydes or ketones † ‡

@inproceedings{Land2016OnepotBA,
  title={One-pot biocatalytic amine transaminase / acyl transferase cascade for aqueous formation of amides from aldehydes or ketones † ‡},
  author={Henrik Land and Peter Hendil-Forssell and Mats Martinelle and Per Berglund},
  year={2016}
}
Biocatalytic acylation/amidation is well-known and has been applied for decades but it has often been limited to the use of organic solvents to avoid hydrolysis. The acyl transferase from Mycobacterium smegmatis (MsAcT) is an enzyme that can perform trans-acylations in aqueous solution. Only a few hydrolases can catalyze trans-acylation in water and MsAcT also has the ability to act as a perhydrolase and catalyze the formation of peracids from esters. Since MsAcT can catalyze acylation for the… CONTINUE READING