One antigen, one gold? A quantitative analysis of immunogold labeling of plasma membrane 5'-nucleotidase in frozen thin sections.

@article{Howell1987OneAO,
  title={One antigen, one gold? A quantitative analysis of immunogold labeling of plasma membrane 5'-nucleotidase in frozen thin sections.},
  author={Kathryn E. Howell and U Reuter-Carlson and Eileen Devaney and J Paul Luzio and Stephen D. Fuller},
  journal={European journal of cell biology},
  year={1987},
  volume={44 2},
  pages={318-27}
}
We present an evaluation of the efficiency of immunogold labeling for a low abundance plasma membrane protein. Several independent methods were used to determine the density of 5'-nucleotidase on the plasma membrane of the Fao cell. These methods include morphometry in combination with either enzymology or cell surface radiometric assay. Immunocytochemistry of frozen thin sections with either single or double layers of antibody and visualized with protein A complexed with 5 nm colloidal gold… CONTINUE READING