One-Way Control of FWA Imprinting in Arabidopsis Endosperm by DNA Methylation

@article{Kinoshita2004OneWayCO,
  title={One-Way Control of FWA Imprinting in Arabidopsis Endosperm by DNA Methylation},
  author={Tetsu Kinoshita and Asuka Miura and Yeonhee Choi and Yuki Kinoshita and Xiaofeng Cao and Steven E. Jacobsen and Robert L. Fischer and Tetsuji Kakutani},
  journal={Science},
  year={2004},
  volume={303},
  pages={521 - 523}
}
The Arabidopsis FWA gene was initially identified from late-flowering epigenetic mutants that show ectopic FWA expression associated with heritable hypomethylation of repeats around transcription starting sites. Here, we show that wild-type FWA displays imprinted (maternal origin–specific) expression in endosperm. The FWA imprint depends on the maintenance DNA methyltransferase MET1, as is the case in mammals. Unlike mammals, however, the FWA imprint is not established by allele-specific de… 
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573 Citations
Highly Influential Citations
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Background Citations
295
Methods Citations
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Results Citations
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573 Citations

Control of FWA gene silencing in Arabidopsis thaliana by SINE-related direct repeats.

The results indicate that methylation of the direct repeats, which presumably originated from a short interspersed nuclear element (SINE), is sufficient to induce proper epigenetic control of the FWA gene.

Maintenance of DNA Methylation during the Arabidopsis Life Cycle Is Essential for Parental Imprinting[W]

It is shown that FWA and FIS2 imprinting requires the maintenance of DNA methylation throughout the plant life cycle, including male gametogenesis and endosperm development, and proposes that imprinting has evolved under constraints linked to the evolution of plant reproduction and not by the selection of a specific molecular mechanism.

DNA LIGASE I exerts a maternal effect on seed development in Arabidopsis thaliana

It is shown that loss-of-function of the major DNA LIGASE I (AtLIG1) in Arabidopsis thaliana causes maternal effects in the endosperm, which is the seed tissue that nurtures embryo development.

Determination of DNA methylation of imprinted genes in Arabidopsis endosperm.

This video protocol reports how to conduct a genetic cross, to isolate endosperm tissue from seeds, and to determine the methylation status by bisulfite sequencing.

Epigenetic variation in the FWA gene within the genus Arabidopsis.

It is shown that variation in FWA expression in field isolates having identical DNA sequences is associated with changes in DNA methylation and may change over time, and it is concluded that methylation of this region is critical for FWA silencing in these species.

Regulation and Flexibility of Genomic Imprinting during Seed Development[W]

Several lines of evidence suggest a potential role for imprinting in the embryo, raising questions as to how imprints are erased and reset from one generation to the next.

Position-dependent effects of cytosine methylation on FWA expression in Arabidopsis thaliana

This work investigates the positional importance of DNA methylation at the FWA (FLOWERING WAGENINGEN) locus in Arabidopsis thaliana and shows that cytosine methylation can be established not only over the well-characterized SINE-derived repeat elements that overlap with the transcription start site, but also in more distal promoter regions.

Evolution and Control of Imprinted FWA Genes in the Genus Arabidopsis

It is shown that the tandem repeat structure is dispensable for the epigenetic silencing of the FWA gene, and SINE-related sequence is sufficient for imprinting, vegetative silencing, and targeting of DNA methylation.

Genomic imprinting in plants: the epigenetic version of an Oedipus complex.

DNA Demethylation and Gene Imprinting in Flowering Plants

From the evolutionary point of view, lines of evidence suggest that both double fertilization and gene imprinting might have coevolved in flowering plants for their reproductive success.
...

43 References

DEMETER, a DNA Glycosylase Domain Protein, Is Required for Endosperm Gene Imprinting and Seed Viability in Arabidopsis

The late flowering phenotype of fwa mutants is caused by gain-of-function epigenetic alleles of a homeodomain gene.

Demethylation-Induced Developmental Pleiotropy in Arabidopsis

Findings implicate DNA methylation in establishing or maintaining epigenetic developmental states in the meristem in both vegetative and reproductive phases of development.

Maintenance of genomic imprinting at the Arabidopsis medea locus requires zygotic DDM1 activity.

The results indicate that the maintenance of the genomic imprint at the mea locus requires zygotic DDM1 activity, and chromatin structure is likely to be interrelated with genomic imprinting in Arabidopsis.

Imprinting of the MEA Polycomb gene is controlled by antagonism between MET1 methyltransferase and DME glycosylase.

Imprinting of the MEDEA Polycomb Gene in the Arabidopsis Endosperm

Results suggest that the embryo abortion observed in mutant mea seeds is due, at least in part, to a defect in endosperm function, which supports the parental conflict theory for the evolution of imprinting in plants and mammals.

Genetic characterization of late-flowering traits induced by DNA hypomethylation mutation in Arabidopsis thaliana.

  • T. Kakutani
  • Biology
    The Plant journal : for cell and molecular biology
  • 1997
Late-flowering traits observed at high frequencies in independently-established ddm1 lines were genetically characterized and provide useful system for identifying epigenetically-regulated genes important for plant development.

Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development.

Observations suggest that DNA methylation plays an important role in regulating many developmental pathways in plants and that the developmental abnormalities seen in the methyltransferase antisense plants may be due to dysregulation of gene expression.

Hypermethylated SUPERMAN epigenetic alleles in arabidopsis.

Seven heritable but unstable sup epi-alleles (the clark kent alleles) are associated with nearly identical patterns of excess cytosine methylation within the SUP gene and a decreased level of SUP RNA.

Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene.

CMT3 is a key determinant for non-CG methylation in animals and the lack of CMT homologs in animal genomes could account for the observation that in contrast to plants, animals maintain primarily CG methylation.