One‐step production of 2,3‐butanediol from starch by secretory over‐expression of amylase in Klebsiella pneumoniae

@inproceedings{Zheng2008OnestepPO,
  title={One‐step production of 2,3‐butanediol from starch by secretory over‐expression of amylase in Klebsiella pneumoniae},
  author={Yu Liang Zheng and Haiyi Zhang and Li Zhao and Liujing Wei and Xingyuan Ma and Dongzhi Wei},
  year={2008}
}
BACKGROUND: 2,3-Butanediol (2,3-BD) is a valuable chemical that can be biosynthesized from many kinds of substrates. For commercial biological production of 2,3-BD, it is desirable to use cheap substrate without pretreatment, such as starch. However, there have been few reports on the production of 2,3-BD directly from starch. RESULTS: In this work, gene malS coding for α-amylase (EC 3.2.1.1) precursor was inserted into plasmid pUC18K, and secretory over-expression of α-amylase was… CONTINUE READING