Oncogenic transformation induced by membrane-targeted Akt2 and Akt3

  title={Oncogenic transformation induced by membrane-targeted Akt2 and Akt3},
  author={Ines Mende and Scott Malstrom and Philip N Tsichlis and Peter K. Vogt and Masahiro Aoki},
The kinases Akt2, Akt3 and their myristylated variants, Myr-Akt2 and Myr-Akt3 were expressed by the RCAS vector in chicken embryo fibroblasts (CEF). Myr-Akt2 and Myr-Akt3 were strongly oncogenic, inducing multilayered foci of transformed cells. In contrast, wild-type Akt2 and Akt3 were only poorly transforming, their efficiencies of focus formation were more than 100-fold lower; foci appeared later and showed less multilayering. Addition of the myristylation signal not only enhanced oncogenic… CONTINUE READING