Oncogenes in solid human tumours

  title={Oncogenes in solid human tumours},
  author={Simonetta Pulciani and Eugenio Santos and Anne V. Lauver and Linda K. Long and Stuart A. Aaronson and Mariano Barbacid},
The identification and isolation of oncogenes capable of inducing malignant transformation on transfection into rodent cells from human tumour cells has opened the possibility of deciphering the processes involved in human carcinogenesis. With the exception of three human lymphomas1, the human transforming genes so far identified have been detected in established tumour cell lines1–8, raising the possibility that they might have been activated during in vitro manipulation of the cells. However… Expand

Paper Mentions

Interventional Clinical Trial
This trial is a multicenter, prospective cohort study aiming to describe molecular profiles of soft tissue sarcoma (STS) with complex genomic profiles in particular to assess the… Expand
ConditionsAdvanced Cancer, Metastatic Cancer, Soft Tissue Sarcoma
Mouse skin carcinomas induced in vivo by chemical carcinogens have a transforming Harvey-ras oncogene
It is reported here that high molecular weight DNA from transplanted squamous cell carcinomas induced by sequential treatment of mouse skin with initiators and promoters of carcinogenesis causes morphological transformation of NIH/3T3 fibroblasts at high frequency. Expand
A novel transforming gene in a human malignant melanoma cell line
The presence of transforming activity in the DNA from a human melanoma cell line which shows weak homology with members of the ras oncogene family is described. Expand
Fibroblast immortality is a prerequisite for transformation by EJ c-Ha-ras oncogene
It is shown that EJ c-Ha-ras-1 lacks complete transforming activity when transfected into normal fibroblasts which have a limited life-span, but can fully transform fibro Blasts that have been newly ‘immortalized’ by carcinogens. Expand
Oncogenes and tumor-suppressor genes.
In addition to studying the pathogenic role of oncogenes, this work is attempting to define negative growth-regulating genes that have tumor-suppressive effects for human lung carcinomas, and involves loss of heterozygosity studies, monochromosome- cell fusion, and cell-cell fusion studies. Expand
Activation of the mouse cellular Harvey-ras gene in chemically induced benign skin papillomas
It is demonstrated that primary papillomas induced by chemical carcinogens in two different mouse strains have an activated c-rasH gene, the first report of a benign tumour which contains DNA with detectable transforming activity. Expand
Activation of a cellular proto-oncogene in spontaneous liver tumor tissue of the B6C3F1 mouse.
Experiments using Southern blot hybridization analysis have identified this active cellular oncogene to be a member of the ras oncGene family and will lead to an increased understanding of potential mechanisms by which hepatic tumors are enhanced and should provide more informed estimates of risk for man. Expand
Oncogene Activation and Expression during Carcinogenesis in Liver and Pancreas
Knowledge of the carcinogenic process has been dramatically increased by the application of recombinant DNA technology and the discovery that mutated cellular genes capable of causing neoplasticExpand
The Role of Oncogenes in Multistage Carcinogenesis
Some oncogenes, for example those belonging to the ras family or the myc gene, have been isolated on several occasions in entirely independent viruses, suggesting that they play a very important role in tumour induction. Expand
The Search for Oncogenes in Breast Cancer
The concept of oncogenes has evolved impressively with the demonstration of the conferral onto cultured cells of a dominant transformed phenotype by tumor cell DNA mediated gene transfer (1–3).Expand
Oncogenes and the Genetic Dissection of Human Cancer: Implications for Basic Research and Clinical Medicine
Of the alterations of cellular oncogenes in human tumors by amplification, base-pair mutation, and rearrangement, respectively, neither mechanism appears to be sufficient to trigger oncogenic transformation, but these alterations can serve as definite markers for the classification of human tumors. Expand


Oncogenes in human tumor cell lines: molecular cloning of a transforming gene from human bladder carcinoma cells.
The results indicate that a biologically active oncogene present in T24 human bladder carcinoma cells is molecularly cloned with lambda Charon 9A as the cloning vector. Expand
Isolation of a transforming sequence from a human bladder carcinoma cell line
The oncogene appears to have undergone little, if any, amplification in several bladder carcinoma cell lines and is unrelated to transforming sequences detected in a variety of other types of human tumor cell lines derived from colonic and lung carcinoma and from neuroblastoma. Expand
Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts
DNAs obtained from human, rabbit and mouse bladder carcinomas lines, a lung carcinoma line and rat neuroblastoma and mouse glioma lines, are able to induce transformation of NIH3T3 cells on transfection. Expand
Transforming genes of human bladder and lung carcinoma cell lines are homologous to the ras genes of Harvey and Kirsten sarcoma viruses.
Results indicate that the transforming genes of these human tumor cell lines are the cellular homologs of two retroviral transforming genes. Expand
T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes
A transforming gene isolated from T24 human bladder carcinoma cells is closely related to the BALB murine sarcoma virus (MSV) onc gene (v-bas), which implies that rather subtle genetic alterations have led to the activation of the normal human homologue of v-bas as a human transforming gene. Expand
Human-tumor-derived cell lines contain common and different transforming genes
The results indicate that overlapping pathways leading to tumorigenesis may arise independently, and that at least three different transforming genes are present in these five lines. Expand
Activation of related transforming genes in mouse and human mammary carcinomas.
  • M. Lane, A. Sainten, G. Cooper
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1981
High molecular weight DNAs of five tumors induced by mouse mammary tumor virus (MMTV), two mouse mammary tumors induced by a chemical carcinogen, and one human mammary tumor cell line (MCF-7) wereExpand
Established cell line of urinary bladder carcinoma (T24) containing tumour‐specific antigen
The permanent presence of tumour‐specific antigen (TSA) in this carcinoma cell line suggests that the TSA is a genetically determined characteristic of T24 cells. Expand
Three different human tumor cell lines contain different oncogenes
Comparison of the common DNA fragments found in secondary foci derived from three different human tumor cell lines indicates that these three cell lines contain three different transforming genes. Expand
Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells
It is found that T24, a cell line derived from a human bladder carcinoma, can induce the morphological transformation of NIH 3T3 cells, and the gene responsible for this transformation is human in origin, <5 kilobase pairs in size and homologous to a 1,100-base polyadenylated RNA species found in T24 and HeLa cells. Expand