On using samples of known protein content to assess the statistical calibration of scores assigned to peptide-spectrum matches in shotgun proteomics.

@article{Granholm2011OnUS,
  title={On using samples of known protein content to assess the statistical calibration of scores assigned to peptide-spectrum matches in shotgun proteomics.},
  author={Viktor Granholm and William Stafford Noble and Lukas K{\"a}ll},
  journal={Journal of proteome research},
  year={2011},
  volume={10 5},
  pages={2671-8}
}
In shotgun proteomics, the quality of a hypothesized match between an observed spectrum and a peptide sequence is quantified by a score function. Because the score function lies at the heart of any peptide identification pipeline, this function greatly affects the final results of a proteomics assay. Consequently, valid statistical methods for assessing the quality of a given score function are extremely important. Previously, several research groups have used samples of known protein… CONTINUE READING