On the properties of alpha-glucosidase and the binding of glucose to the enzyme.

@article{Siro1978OnTP,
  title={On the properties of alpha-glucosidase and the binding of glucose to the enzyme.},
  author={M R Siro and Timo L{\"o}vgren},
  journal={Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry},
  year={1978},
  volume={32 6},
  pages={447-51}
}
An alpha-glucosidase was purified from baker's yeast. The molecular weight was approximately 44 000 daltons. SDS-disc gel electrophoresis suggested that the enzyme consisted of four subunits. The isoelectric point was at pH 5.4. The Km values for p-nitrophenyl alpha-D-glucopyranoside and maltose were 2.9 X 10(-4) and 2.5 X 10(-2) M, respectively. Binding of… CONTINUE READING