On the freezing and identification of lipid monolayer 2-D arrays for cryoelectron microscopy.

@article{Taylor2007OnTF,
  title={On the freezing and identification of lipid monolayer 2-D arrays for cryoelectron microscopy.},
  author={Dianne W. Taylor and Deborah F Kelly and Anchi Cheng and Kenneth A Taylor},
  journal={Journal of structural biology},
  year={2007},
  volume={160 3},
  pages={
          305-12
        }
}
Lipid monolayers provide a convenient vehicle for the crystallization of biological macromolecules for 3-D electron microscopy. Although numerous examples of 3-D images from 2-D protein arrays have been described from negatively stained specimens, only six structures have been done from frozen-hydrated specimens. We describe here a method that makes high quality frozen-hydrated specimens of lipid monolayer arrays for cryoelectron microscopy. The method uses holey carbon films with patterned… CONTINUE READING

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