On the formation of spontaneous deletions: The importance of short sequence homologies in the generation of large deletions

@article{Albertini1982OnTF,
  title={On the formation of spontaneous deletions: The importance of short sequence homologies in the generation of large deletions},
  author={Alessandra M. Albertini and Murielle Hofer and Mich{\'e}le P. Calos and Jeffrey H. Miller},
  journal={Cell},
  year={1982},
  volume={29},
  pages={319-328}
}
Using lacl-Z fusion strains of Escherichia coli we have devised systems that detect deletions of varying lengths. We examined deletions 700-1000 base pairs long, and genetically characterized over 250 spontaneous deletions. Of these, we analyzed 24 by direct DNA sequencing and 18 by inspection of restriction fragment patterns. Deletions of this size occur almost exclusively at short repeated sequences in both (recA+ and recA- strain backgrounds, but are detected 25-fold more frequently in a… 
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TLDR
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