On the fluorometric assay of circulating lipoperoxides.

  title={On the fluorometric assay of circulating lipoperoxides.},
  author={Thijs Hendriks and Ralph Assmann},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  volume={174 3},
The methodology for the measurement of circulating thiobarbituric acid (TBA)-reactive products of lipid peroxidation in human plasma by means of fluorometry has been reinvestigated. The lipid precipitation of plasma with phosphotungstic acid, which step is laid down in the standard assay method, strongly increases the apparent TBA-reactivity. However, subsequent washing of the precipitate may reduce the apparent levels of lipoperoxide to values close to those obtained by using untreated plasma… CONTINUE READING