On the concentrations of cyclins and cyclin-dependent kinases in extracts of cultured human cells.

@article{Arooz2000OnTC,
  title={On the concentrations of cyclins and cyclin-dependent kinases in extracts of cultured human cells.},
  author={T. Arooz and C. Yam and W. Y. Siu and A. Lau and K. K. Li and R. Poon},
  journal={Biochemistry},
  year={2000},
  volume={39 31},
  pages={
          9494-501
        }
}
Cyclins and cyclin-dependent kinases (CDKs) are key regulators of the human cell cycle. Here we have directly measured the concentrations of the G(1) and G(2) cyclins and their CDK partners in highly synchronized human cervical carcinoma cells (HeLa). To determine the exact concentrations of cyclins and CDKs in the cell extracts, we developed a relatively simple method that combined the use of (35)S-labeled standards produced in rabbit reticulocyte lysates and immunoblotting with specific… Expand
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