On the chemistry of formaldehyde fixation and its effects on immunohistochemical reactions

  title={On the chemistry of formaldehyde fixation and its effects on immunohistochemical reactions},
  author={Holde Puchtler and Susan N. Meloan},
Formalin has been recommended as an innocuous fixative for immunohistochemistry. However, several studies demonstrated impairment or blocking of antigenic activity of certain proteins. Formalin fixation was discovered accidentally by F. Blum in 1893 and its deleterious effects on various tissue structures were discussed extensively during the following decades. More recently, some authors assumed that formaldehyde bound to tissues can be largely or completely removed by washing and dehydration… CONTINUE READING

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