On the cell-free association of lamins A and C with metaphase chromosomes.

@article{Burke1990OnTC,
  title={On the cell-free association of lamins A and C with metaphase chromosomes.},
  author={Bernard P. Burke},
  journal={Experimental cell research},
  year={1990},
  volume={186 1},
  pages={169-76}
}
Nuclear envelopes have previously been shown to assemble spontaneously around endogenous chromosomes in cell-free homogenates of mitotic Chinese hamster ovary cells. In order to further analyze the mechanisms underlying nuclear envelope reformation and the functions of the individual nuclear lamin polypeptides, a fractionated cell-free nuclear envelope reassembly system involving purified chromosomes and either a postchromosomal supernatant or a cytosol fraction from mitotic cells has been… CONTINUE READING

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