On the binding of iabelled saxitoxin to the squid giant axon

  title={On the binding of iabelled saxitoxin to the squid giant axon},
  author={Richard Darwin Keynes and Joseph Murdoch Ritchie},
  journal={Proceedings of the Royal Society of London. Series B. Biological Sciences},
  pages={147 - 153}
  • R. Keynes, J. M. Ritchie
  • Published 1984
  • Chemistry, Medicine
  • Proceedings of the Royal Society of London. Series B. Biological Sciences
Measurement of the maximum saturable binding capacity for 3H-labelled saxitoxin in well cleaned giant axons from Loligo forbesi gave a value of 290 ± 65 (s. e. m.) binding sites per square micrometre of membrane. In samples of the fin nerve the total saturable uptake was 12.4 ± 2.4 fmol mg-1 wet mass, corresponding to 94 sites per square micrometre for fibres whose mean diameter after fixation and embedding was estimated as 16 µm. 
Binding of [3H]saxitoxin to the voltage-dependent Na channels and inhibition of 22Na influx in bovine adrenal medullary cells
Results indicate that saxitoxin binds to a specific site on voltage-dependent Na channels and inhibits the influx of 22Na, and would be useful for the detailed analysis of voltage- dependent Na channels in adrenal medullary cells. Expand
Tetrodotoxin —A brief history—
  • T. Narahashi
  • Chemistry, Medicine
  • Proceedings of the Japan Academy. Series B, Physical and biological sciences
  • 2008
The history of discovery of TTX action on sodium channels is described, and the TTX-resistant sodium channels have been discovered in the nervous system and received much attention because of their role in pain sensation. Expand
A slow component in the gating current of the frog node of Ranvier
The slow component of the gating current on-response has been studied on voltage-clamped nodes of Ranvier of the frog Rana esculenta and most likely reflects charge movement between different open states; it does not seem to be related to inactivation of the sodium channels or activation of the potassium channels. Expand
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A new method for quantitating NSP toxins using the synaptoneurosomal technique was developed and a strong relationship between STX added and STX detected revealed, indicating that veratrine represents a valid alternative to veratridine in this assay. Expand
The evolution and origin of tetrodotoxin acquisition in the blue-ringed octopus (genus Hapalochlaena).
This review focuses on the evolution of tetrodotoxin acquisition, in particular how it may have occurred within the blue-ringed octopus genus (Hapalochlaena) and the subsequent impact on venom evolution. Expand
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Distribution of Saxitoxin‐binding Sites in Mammalian Neural Tissue
  • J. M. Ritchie
  • Chemistry, Medicine
  • Annals of the New York Academy of Sciences
  • 1986


The binding of tritiated tetrodotoxin to squid giant axons.
  • S. Levinson, H. Meves
  • Medicine, Biology
  • Philosophical transactions of the Royal Society of London. Series B, Biological sciences
  • 1975
The binding of tetrodotoxin to squid gian axons was determined as a function of toxin concentration, using a tritiated toxin preparation of known radiochemical purity and specific activity to identify toxin binding sites thought to be sodium channels. Expand
The rate of action of tetrodotoxin on sodium conductance in the squid giant axon.
The figure obtained for the number of sodium channels in the squid giant axon, several hundred per square micrometre, agrees well with those derived from other lines of argument. Expand
Sodium channels in the axolemma of unmyelinated axons: a new estimate
The saturable uptake of tritium-labeled saxitoxin by the rat left cervical sympathetic trunk, which consists almost entirely of unmyelinated fibers, indicates that the density of sodium channels onExpand
The binding of labelled saxitoxin to the sodium channels in normal and denervated mammalian muscle, and in amphibian muscle.
The binding of saxitoxin to innervated and denervated rat diaphragm muscle, and to normal frog muscle, has been measured and it is suggested that the decrease in density per unit weight does not reflect any change in the density of sodium channels per unit area of membrane. Expand
A new method for labelling saxitoxin and its binding to non‐myelinated fibres of the rabbit vagus, lobster walking leg, and garfish olfactory nerves.
A new method of labelling saxitoxin (STX) is described, based on transfer of tritium from tritiated water to the toxin, an improvement of about 300‐fold over other means oflabelling TTX and STX. Expand
The kinetics of the interaction between tetrodotoxin and mammalian nonmyelinated nerve fibers.
It has been shown that the rate of action of tetrodotoxin may well be controlled by diffusion (slowed by simultaneous adsorption) rather than by the rates of the drug-receptor interaction. Expand
The interaction at equilibrium between tetrodotoxin and mammalian non‐myelinated nerve fibres
An estimate has been made of the equilibrium dissociation constant (K) for the interaction of tetrodotoxin (TTX) with the non‐myelinated fibres of the rabbit desheathed vagus nerve. Expand
The conductance of sodium channels in the squid giant axon
  • J . Physiol . , Lond .
  • 1983
Binding of radioactively labelled saxitoxin to the squid giant axon
  • J . Membrane Biol
  • 1979
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