On the activity loss of hydrolases in organic solvents: II. a mechanistic study of subtilisin Carlsberg

@article{Castillo2006OnTA,
  title={On the activity loss of hydrolases in organic solvents: II. a mechanistic study of subtilisin Carlsberg},
  author={B. Castillo and Vibha Bansal and Ashok Ganesan and Peter James Halling and Francesco Secundo and Amaris Ferrer and Kai Griebenow and Gabriel L Barletta},
  journal={BMC Biotechnology},
  year={2006},
  volume={6},
  pages={51 - 51}
}
BACKGROUND Enzymes have been extensively used in organic solvents to catalyze a variety of transformations of biological and industrial significance. It has been generally accepted that in dry aprotic organic solvents, enzymes are kinetically trapped in their conformation due to the high-energy barrier needed for them to unfold, suggesting that in such media they should remain catalytically active for long periods. However, recent studies on a variety of enzymes demonstrate that their initial… CONTINUE READING