On the activities of Escherichia coli exonuclease III.

  title={On the activities of Escherichia coli exonuclease III.},
  author={Jorg D Hoheisel},
  journal={Analytical biochemistry},
  volume={209 2},
Exonuclease III (Exo III) of Escherichia coli is a DNA-modifying enzyme very frequently used in molecular biology. The experiments described here were carried out with the aims of reliably controlling exonuclease activity and of learning more about the enzyme's specificities. The dependence of Exo III activity on factors such as temperature (including heat inactivation), the concentrations of Exo III and NaCl, and the concentration and shape of 3' termini was investigated. Double stranded DNA… CONTINUE READING

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