On-line monitoring of apoptosis in insulin-secreting cells.

  title={On-line monitoring of apoptosis in insulin-secreting cells.},
  author={Martin K{\"o}hler and Sergei V. Zaitsev and Irina I. Zaitseva and Barbara Leibiger and Ingo B. Leibiger and Mikael Turunen and Iouri L. Kapelioukh and Linda Bakkman and Ioulia B. Appelskog and Jacques Boutet de Monvel and Gabriela Imreh and P. -O. Berggren},
  volume={52 12},
Apoptosis was monitored in intact insulin-producing cells both with microfluorometry and with two-photon laser scanning microscopy (TPLSM), using a fluorescent protein based on fluorescence resonance energy transfer (FRET). TPLSM offers three-dimensional spatial information that can be obtained relatively deep in tissues. This provides a potential for future in vivo studies of apoptosis. The cells expressed a fluorescent protein (C-DEVD-Y) consisting of two fluorophores, enhanced cyan… CONTINUE READING
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Online monitoring of apoptosis in living pancreatic beta-cells (Abstract)

  • SV Zaitsev, M Köhler, +5 authors PO Berggren
  • Diabetologia 45 (Suppl
  • 2002
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