On-line identification of secondary metabolites in freshwater microalgae and cyanobacteria by combined liquid chromatography-photodiode array detection-mass spectrometric techniques.

@article{Frassanito2005OnlineIO,
  title={On-line identification of secondary metabolites in freshwater microalgae and cyanobacteria by combined liquid chromatography-photodiode array detection-mass spectrometric techniques.},
  author={Rita Frassanito and Marco Cantonati and M T Tard{\'i}o and Ines Mancini and Graziano Guella},
  journal={Journal of chromatography. A},
  year={2005},
  volume={1082 1},
  pages={
          33-42
        }
}
The analysis and identification of a wide range of secondary metabolites biosynthesized by different algal taxa and cyanobacteria has been performed through a selective and sensitive methodology, mainly based on reversed-phase HPLC coupled both to UV photodiode array detection and to atmospheric pressure mass spectrometric techniques (HPLC-DAD-APIMS). Results are reported here with special attention to the analyses carried out both on the natural phytoplankton (mixed populations) of Lake Tovel… Expand
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References

SHOWING 1-10 OF 19 REFERENCES
QUANTIFICATION OF THE RELATIVE ABUNDANCE OF THE TOXIC DINOFLAGELLATE, KARENIA BREVIS (DINOPHYTA), USING UNIQUE PHOTOPIGMENTS 1
TLDR
Measurements of gyroxanthin‐diester and other diagnostic pigments of Karenia brevis were incorporated into the initial pigment ratio matrix of the chemical taxonomy program (CHEMTAX) to resolve the relative contribution of K. brevis biomass in mixed estuarine phytoplankton assemblages from Florida and Galveston Bay, Texas. Expand
Microalgal Pigment Assessments Using High-Performance Liquid Chromatography: A Synopsis of Organismal and Ecological Applications
TLDR
HPLC can accurately characterize phylogenetic groups and changes in community composition and yield information concerning microalgal physiological status, production, trophic interaction, and paleolimnology/paleooceanography. Expand
A new solution for an old problem: the regiochemical distribution of the acyl chains in galactolipids can be established by electrospray ionization tandem mass spectrometry.
TLDR
Electrospray ionization-quadrupole ion trap mass spectrometry (ESI-QITMS), either in positive- or in negative-ion mode, has been used to establish the chemical structures of the fatty acids attached to the primary and secondary hydroxyl groups of the glycerol moiety of natural monogalactosyl- (MGDG) and DGDG mixtures. Expand
The synthesis of mycosporine-like amino acids (MAAs) by cultured, symbiotic dinoflagellates.
TLDR
The hypothesis that there is a relation between phylotypes (phylogenetic types) and the synthesis of mycosporine-like amino acids by symbiotic dinoflagellates under the influence of ultraviolet radiation and photosynthetically active radiation is tested. Expand
Liquid chromatography/mass spectrometry of carotenoids
The sensitivity, specificity and selectivity of liquid chromatography/mass spectrometry (LC/MS) and LC-tandem mass spectrometry (LC/MS/MS) make these essential tools for the characterization andExpand
Photoprotective compounds in cyanobacteria, phytoplankton and macroalgae--a database.
TLDR
The database answers the urgent need for a library on these substances and provides scientists in the field with the necessary information to identify and quantify screening pigments in different aquatic organisms from various growing sites and different collection dates. Expand
CHEMTAX - a program for estimating class abundances from chemical markers: application to HPLC measurements of phytoplankton
TLDR
A new program for calculating algal class abundances from measurements of chlorophyll and carotenoid pigments determined by high-performance liquid chromatography (HPLC) that can provide good estimates of prochlorophytes, even in the absence of experimental data on the concentrations of divinyl-chlorophylls a and b. Expand
Blooms of the dinoflagellate Glenodinium sanguineum obtained during enclosure experiments in Lake Tovel (N. Italy)
Freshwater red tides due to dinoflagellates are less common than their marine analogues, which are usually a serious problem and can even be toxic. This was not the case for Lake Tovel in theExpand
Mycosporine-like amino acids (MAAs) in phytoplankton, a herbivorous pteropod (Limacinahelicina), and its pteropod predator (Clioneantarctica) in McMurdo Bay, Antarctica
TLDR
Findings point to the potential for MAAs (and thereby UV-photoprotection) to be transferred, not just from primary producers to primary consumers, but also to the second trophic level of secondary consumers. Expand
The structure of scytonemin, an ultraviolet sunscreen pigment from the sheaths of cyanobacteria
TLDR
The pigment scytonemin, the first shown to be an effective, photo-stable ultraviolet shield in prokaryotes, is a novel dimeric molecule of indolic and phenolic subunits and is known only from the sheaths enclosing the cells of cyanobacteria. Expand
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