On-line estimation of the biomass activity during animal-cell cultivations.

  title={On-line estimation of the biomass activity during animal-cell cultivations.},
  author={R. C. Dorresteijn and K H Numan and C D de Gooijer and Johannes Tramper and E. Coen Beuvery},
  journal={Biotechnology and bioengineering},
  volume={51 2},
A software sensor was developed to determine the volumetric biomass activity of animal cell cultivations on-line. It was based on the on-line estimation of the ATP-production rate from the oxygen uptake and the lactic-acid production rate. The sensor was verified for a batch culture of Vero cells, and a batch and a continuous culture of hybridoma cells. For the hybridoma cells, the sensor showed a good correlation with the biomass concentration. However, this was not the case for the Vero cells… CONTINUE READING