On-line enzymatic amplification by substrate cycling in a dual bioreactor with rotation and amperometric detection.

@article{Raba1994OnlineEA,
  title={On-line enzymatic amplification by substrate cycling in a dual bioreactor with rotation and amperometric detection.},
  author={Julio Raba and Horacio A. Mottola},
  journal={Analytical biochemistry},
  year={1994},
  volume={220 2},
  pages={297-302}
}
The amplification approach centered on the cycling of two reversibly interconvertible chemical species sequentially participating in two different enzyme-catalyzed reactions (enzymatic amplification by substrate cycling) has been implemented on-line into a continuous-flow/stopped-flow/continuous-flow operation. The implementation is illustrated with the determination of L-lactate in a dual enzyme reactor containing immobilized lactate oxidase (LOD, EC 1.1.3.x) to catalyze the oxidation of L… CONTINUE READING

Citations

Publications citing this paper.

Similar Papers

Loading similar papers…