On-chip synthesis of RNA aptamer microarrays for multiplexed protein biosensing with SPR imaging measurements.

Abstract

Microarrays of RNA aptamers are fabricated in a one-step, multiplexed enzymatic synthesis on gold thin films in a microfluidic format and then employed in the detection of protein biomarkers with surface plasmon resonance imaging (SPRI) measurements. Single-stranded RNA (ssRNA) oligonucleotides are transcribed on-chip from double-stranded DNA (dsDNA… (More)
DOI: 10.1021/la300656c

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