On Experimental Artifacts in the Use of Metal Ion Chelators for the Determination of the Cation Binding Constants of a-Lactalbumin

The binding constant of Ca2+ to the strong cation site of bovine a-lactalbumin has been measured directly by monitoring the free calcium concentration by Quin 2 fluorescence. A dissociation constant of 1-4 nM was calculated, which confirms the strong calcium binding properties of this protein. In order to examine whether the metal ion chelators EDTA or EGTA… CONTINUE READING