Omp85 genosensor for detection of human brain bacterial meningitis

Abstract

The 5′-thiolated DNA probe based on specific virulence gene, Omp85, was immobilized onto a screen-printed gold electrode followed by hybridization with 6–100 ng/6 μl (5.9 × 105–9.3 × 106 c.f.u.) of Neisseria meningitidis single stranded genomic DNA (ssG-DNA) for 10 min at 25 °C from the cerebrospinal fluid (CSF) of a meningitis patient. The Omp85 genosensor… (More)
DOI: 10.1007/s10529-013-1161-2

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