• Corpus ID: 40782031

Omasal , Reticular Sampling Techniques , and Dual-flow Continuous Culture System for Assessing Ruminal Digestion , Nutrient , and Microbial Protein Flow out of the Rumen and Canola Meal as a Protein Supplement for Lactating Dairy Cows

  title={Omasal , Reticular Sampling Techniques , and Dual-flow Continuous Culture System for Assessing Ruminal Digestion , Nutrient , and Microbial Protein Flow out of the Rumen and Canola Meal as a Protein Supplement for Lactating Dairy Cows},
  author={Antonio Pinheiro Faciola},
This paper is divided in three components: 1) Comparing the omasal and the reticular sampling techniques; 2) Elaborating on the dual-flow continuous culture system; and 3) Summarizing recent studies on canola meal as the main protein supplement for lactating dairy cows. The goal is to illustrate some peculiarities among these three different methodologies and to point out recent findings on canola meal research. 



Determination of reticulo-rumen and whole-stomach digestion in lactating cows by omasal canal or duodenal sampling

The results indicated that the omasal sampling technique provides a promising alternative to the duodenal sampling techniques to investigate forestomach digestion in dairy cows and offers an alternative means to study rumen N metabolism.

Comparative characterization of reticular and duodenal digesta and possibilities of estimating microbial outflow from the rumen based on reticular sampling in dairy cows.

  • A. Hristov
  • Biology, Medicine
    Journal of animal science
  • 2007
The concept that microbial protein outflow from the rumen can be measured based on sampling of ruminal or reticular digesta is supported, and the reticular sampling technique can also provide reliable estimates for ruminal digestibility of OM, N, and fiber fractions.

Omasal sampling technique for assessing fermentative digestion in the forestomach of dairy cows.

The omasal sampling procedure requires less surgical intervention than the traditional methods using abomasal or duodenal cannulas as sampling sites to study forestomach digestion and avoids potentially confounding endogenous secretions of the abomasum.


The reticulo-omasal orifice was not a site of discrimination of the size of digesta particles that leave the rumen, and large particles could clearly pass through the orifice.

A meta-analysis of variability in continuous-culture ruminal fermentation and digestibility data.

This analysis showed that CC systems are generally characterized by lower total VFA and acetate concentrations, extremely low counts or lack of ruminal protozoa, and lower organic matter and NDF digestibilities than in vivo.

Quantifying ruminal nitrogen metabolism using the omasal sampling technique in cattle--a meta-analysis.

Improved efficiency of microbial-N synthesis and reduced ruminal CP degradability were positively associated with efficiency of capture of dietary N as milk N and the omasal sampling technique yields valuable estimates of RDP, RUP, and ruminal microbial protein supply in cattle.

Physical characteristics of reticular content in the bovine and consequences on reticular outflow.

Reticular content was sampled in cows before and 2 and 5 hours after they were fed various forages and may be autoregulated by adjustment of feeding parameters in response to sensory receptor signals, which helps prolong fermentation time.

Measurement of volatile fatty acid disappearance and fluid flux across the abomasum of cattle, using an improved omasal cannulation technique.

Six steer calves, surgically fitted with a permanent cannula in the rumen, omasoabomasal orifice, abomasum, and duodenum were used to determine total digesta flow and volatile fatty acid (VFA)

Effects of different protein supplements on milk production and nutrient utilization in lactating dairy cows.

Overall, production and N utilization were compromised when the diets of high-yielding dairy cows were supplemented with urea rather than true protein and the value of the true proteins.

Replacing dietary soybean meal with canola meal improves production and efficiency of lactating dairy cows.

Interactions of parity × protein source and parity × CP concentration indicated that primiparous cows were more responsive than multiparous dairy cows to improved supply of metabolizable protein.