Oligosaccharides synthesis by free and immobilized β-galactosidases from Thermus aquaticus YT-1

@article{Berger2004OligosaccharidesSB,
  title={Oligosaccharides synthesis by free and immobilized β-galactosidases from Thermus aquaticus YT-1},
  author={J. Lee Berger and Byong Hoon Lee and C{\'e}line Lacroix},
  journal={Biotechnology Letters},
  year={2004},
  volume={17},
  pages={1077-1080}
}
Galactooligosaccharides (GalOS) production from a lactose solution (16% w/v) was studied at 70°C, pH 4.6 and 6.0 with free or immobilized β-galactosidases of Thermus aquaticus YT-1. Synthesis of OS and lactose conversion was significantly improved when using the immobilized preparation. At pH 4.6, OS accounted for approx. 35% of the total carbohydrates with a conversion rate of lactose of 80%. The general formula of OS synthesized in this way was Gal-(Gal)n-Glu with n being equal to 1 or 2 and… CONTINUE READING

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