Oligonucleotide probe for the visualization of Escherichia coli/Escherichia fergusonii cells by in situ hybridization: specificity and potential applications.

@article{Regnault2000OligonucleotidePF,
  title={Oligonucleotide probe for the visualization of Escherichia coli/Escherichia fergusonii cells by in situ hybridization: specificity and potential applications.},
  author={B{\'e}atrice Regnault and Sylvie Martin-Delautre and Monique Lejay-Collin and Martine Lef{\`e}vre and Patrick A D Grimont},
  journal={Research in microbiology},
  year={2000},
  volume={151 7},
  pages={521-33}
}
There are several occasions when enumeration of Escherichia coli cells is needed. These include examination of urine specimens and water or food samples. Present methods rely on growth in more or less selective media (colony-forming units on agar or the most probable number method using liquid media). Unfortunately, no really selective medium with 100% efficiency of plating is available for E. coli. A 24-mer oligonucleotide probe (Colinsitu), complementary to a piece of 16S ribosomal… CONTINUE READING
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