Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology.

@article{Guschin1997OligonucleotideMA,
  title={Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology.},
  author={D Y Guschin and Bruce Mobarry and D Proudnikov and David A. Stahl and Bruce E. Rittmann and A. D. Mirzabekov},
  journal={Applied and environmental microbiology},
  year={1997},
  volume={63 6},
  pages={2397-402}
}
The utility of parallel hybridization of environmental nucleic acids to many oligonucleotides immobilized in a matrix of polyacrylamide gel pads on a glass slide (oligonucleotide microchip) was evaluated. Oligonucleotides complementary to small-subunit rRNA sequences of selected microbial groups, encompassing key genera of nitrifying bacteria, were shown to selectively retain labeled target nucleic acid derived from either DNA or RNA forms of the target sequences. The utility of varying the… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 121 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 31 references

Application of phylogenetically based hybridization probes to microbial ecology

  • D. A. Stahl
  • 1995

In situ identification of ammonia-oxidizing bacteria

  • M. Wagner, G. Rath, R. Amann, H.-P. Koops, K.-H. Schleifer
  • Syst. Appl. Microbiol
  • 1995

Similar Papers

Loading similar papers…