Oligonucleotide-directed gene-editing technology: mechanisms and future prospects.

  title={Oligonucleotide-directed gene-editing technology: mechanisms and future prospects.},
  author={Ioannis Papaioannou and J Paul Simons and James Stuart Owen},
  journal={Expert opinion on biological therapy},
  volume={12 3},
INTRODUCTION Gene editing, as defined here, uses short synthetic oligonucleotides to introduce small, site-specific changes into mammalian genomes, including repair of genetic point mutations. Early RNA-DNA oligonucleotides (chimeraplasts) were problematic, but application of single-stranded all-DNA molecules (ssODNs) has matured the technology into a reproducible tool with therapeutic potential. AREAS COVERED The review illustrates how gene-editing mechanisms are linked to DNA repair systems… CONTINUE READING