Oligonucleotide Sequences Required for Natural Killer Cell Activation

@article{Kuramoto1992OligonucleotideSR,
  title={Oligonucleotide Sequences Required for Natural Killer Cell Activation},
  author={Etsuro Kuramoto and Osamu Yano and Y Kimura and Makoto Baba and Tadashi Makino and Saburo Yamamoto and Toshiko Yamamoto and Tetsuro Kataoka and Tohru Tokunaga},
  journal={Japanese Journal of Cancer Research : Gann},
  year={1992},
  volume={83},
  pages={1128 - 1131}
}
Based on the previous finding that certain 30‐mer single‐stranded oligodeoxyribonucleotides (oligonucleotides) having particular 6‐mer palindromic sequences could induce interferon‐alpha and ‐gamma, and enhance natural killer activity, the present study was carried out to clarify the entire relationship between the activity and the sequence of 30‐mer oligonucleotides. The results indicated that the activity depended critically on the presence of particular palindromic sequences including the 5… 
Ability of oligonucleotides with certain palindromes to induce interferon production and augment natural killer cell activity is associated with their base length.
TLDR
It is indicated that the immunostimulatory activity of oligonucleotides with certain palindromic sequences requires an oligon nucleotide at least 18 bases long.
Binding of oligoguanylate to scavenger receptors is required for oligonucleotides to augment NK cell activity and induce IFN.
TLDR
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Hexamer palindromic oligonucleotides with 5'-CG-3' motif(s) induce production of interferon.
TLDR
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Lipofection of Synthetic Oligodeoxyribonucleotide Having a Palindromic Sequence of AACGTT to Murine Splenocytes Enhances Interferon Production and Natural Killer Activity
TLDR
The results strongly suggest that the prerequisite for IFN production is not the binding of AAC‐22 to the cell surface receptors, but its penetration into the spleen cells.
Characterization and modulation of immune stimulation by modified oligonucleotides.
TLDR
One of the most potent ODNs was shown to activate NK and B lymphocytes when administered in vivo, and it was found that phosphorothioate backbones were, in general, less stimulatory than phosphodiester backbones.
Novel chimeric immunomodulatory compounds containing short CpG oligodeoxyribonucleotides have differential activities in human cells.
TLDR
CICs could be 'programmed' for IFN-alpha production or B cell activation as independent variables, and identification of the optimal motifs, spacers and structures for different biological properties allows for the assembly of CICs exhibiting a defined set of activities tailored for specific clinical applications.
Historical Perspectives
TLDR
The response of higher animals against immunostimulatory DNA must be the most primitive but important mechanism for self-nonself discrimination against foreign DNA.
Immunostimulatory DNA sequences : an overview.
TLDR
There have now been several reports that bacterial DNA or synthetic oligodeoxyribo-nucleotides containing bacterially derived sequences, stimulate the immune systems of mice and humans to first mount innate, and then antigen-specific (when foreign antigen is present), Th(1)-type responses.
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