Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays

@article{Maeder2009OligomerizedPE,
  title={Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays},
  author={Morgan L. Maeder and Stacey Thibodeau-Beganny and Jeffry D. Sander and Daniel F. Voytas and J. Keith Joung},
  journal={Nature Protocols},
  year={2009},
  volume={4},
  pages={1471-1501}
}
Engineered zinc-finger nucleases (ZFNs) form the basis of a broadly applicable method for targeted, efficient modification of eukaryotic genomes. In recent work, we described OPEN (oligomerized pool engineering), an 'open-source,' combinatorial selection-based method for engineering zinc-finger arrays that function well as ZFNs. We have also shown in direct comparisons that the OPEN method has a higher success rate than previously described 'modular-assembly' methods for engineering ZFNs. OPEN… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 89 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 82 references

Similar Papers

Loading similar papers…